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calcein cobalt loading procedure  (MedChemExpress)


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    Structured Review

    MedChemExpress calcein cobalt loading procedure
    Calcein Cobalt Loading Procedure, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+cobalt+loading+procedure/Calcein/pm33510088-57-15-25
    Average 94 stars, based on 19 article reviews
    calcein cobalt loading procedure - by Bioz Stars, 2026-10
    94/100 stars

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    Article Title: Mitochonic acid 5 regulates mitofusin 2 to protect microglia
    Article Snippet: Cells were gently washed with PBS, then incubated with 10 mg/mL MitoProbe JC-1 (Cat# C2006, Beyotime Institute of Biotechnology) in the dark for about 30 minutes at 37°C. .. A mitochondrial permeability transition pore (mPTP) opening assay was performed as described, using a well-established calcein cobalt loading procedure with a calcein-acetoxymethyl ester (Cat# HY-D0041; MedChemExpress, Monmouth Junction, NJ, USA) (Tallman et al., 2017). .. After three 5 minute washes in PBS, cells were incubated with a solution containing 2 μM calcein-acetoxymethyl ester and 8 μM cobalt chloride (Cat# H9060; Solarbio Science & Technology Co.) for more than 30 minutes in the dark.

    Article Title: Mitochonic acid 5 regulates mitofusin 2 to protect microglia.
    Article Snippet: Detection of mitochondrial membrane potential and the rate of mPTP opening Cells were gently washed with PBS, then incubated with 10 mg/mL MitoProbe JC-1 (Cat# C2006, Beyotime Institute of Biotechnology) in the dark for about 30 minutes at 37°C. .. A mitochondrial permeability transition pore (mPTP) opening assay was performed as described, using a well-established calcein cobalt loading procedure with a calcein-acetoxymethyl ester (Cat# HY-D0041; MedChemExpress, Monmouth Junction, NJ, USA) (Tallman et al., 2017). .. After three 5 minute washes in PBS, cells were incubated with a solution containing 2 μM calcein-acetoxymethyl ester and 8 μM cobalt chloride (Cat# H9060; Solarbio Science & Technology Co.) for more than 30 minutes in the dark.



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